It is very important to establish a kind of simple and useful marking method to evaluate multiplication and releasing effect and the current situation of fisheries resource. The golden cuttlefish were chosen to be the study matieral in this article. The larval golden cuttlefish hached after 15d were immersed in 6.0 ~ 8.0 ×10^-3 % alizarin complexone (ALC) for 24 h. The tagged group and control group were reared in1.5m×1.5 m×1.5 m indoor pond for 30 d, and then they were removed to 2667 m² outdoor pond. After 60d, their mean doral mantle length grew to 91.4 mm (control group) and 87.6 mm (tagged group) respectively, they were removed to indoor pond for over winter. During 210d experiment, the golden cuttlefish were sampled randomly in order to examine the growth and the dye-retention of the tagged cuttlebone after tagged 15、30、45、60、90、210 d. The results of the experiment showed that this marking method is very effective, and the survival rate of the tagged golden cuttlefish is 100%. There are no significant differences between the growth of the tagged group and the control group (P>0.05). The half tagged lavender ring is very clear in the cuttlebone even after tagged 210d. Additionally, according to this method, we could tag lots of samples easily in one time, and the retention of marker can last long period. Due to the maneuverability and easy to distinguish tagged ones, it is suggested that this method could be a good marking method for golden cuttlefish.