坛紫菜诱变育种的初步研究
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国家自然科学基金项目(40676077)


Preliminary study on mutation breeding of
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    摘要:

    近十几年来,坛紫菜品种退化、产量降低、质量下降,本实验通过诱变处理,筛选和培育优质高产的坛紫菜新品系。坛紫菜野生丝状体经一定剂量60Co-γ射线辐照后,子代叶状体发生了变异,经大量培养后筛选出紫色突变体(3号Ⅰ)和经济性状优良的个体(7号Ⅰ和7号Ⅱ),通过体细胞酶解和单克隆技术快速获得诱变选育纯系,并对其主要经济性状进行了研究。其主要特征:①7号Ⅰ:叶片宽(2.99±0.61)cm、生长快(30 cm以上的藻体长度平均日增长量可达6.51 cm,比对照组快55.3%)、耐高温(29 ℃正常生长,比对照组高2 ℃以上)、总藻胆蛋白含量高(104.86 mg·g-1干品,比对照组高39.0%);②7号Ⅱ:藻体窄,生长快(长度30 cm以上的藻体平均日增长量可达4.26 cm,比对照组快58.7%)、叶片薄(藻体中部厚度仅为22.5~27.5 μm,比对照组薄29.6%)、总藻胆蛋白含量高达104.24 mg·g-1干品,比对照组高39.4%);③3号Ⅰ:藻体紫色;叶片薄,藻体中部厚25.0~32.5 μm;④7号I和7号Ⅱ在生产上应用,产量分别达309.6kg·666.6 m-2和291.5 kg·666.6 m-2,比对照组高37%和29%。本研究结果为坛紫菜种质改良以及良种的开发利用奠定了良好基础。

    Abstract:

    Porphyra haitanensis has degenerated in breed and has a reduced outcome and descent quality recent ten years. The blades were guided to variation in order to select and cultivate new strains of Porphyra haitanensis that were high quality and high yield. 60Co-γray was a useful mutagen in induced mutation breeding .After treated with 60Co-γray ,free-living conchocelis of wild Porphyra haitanensis were induced and represented in offsprings. Purple mutant and excellent practical characters strains were selected from a lot of gametophytic blades. Single cell clone and enzymolysis were used to propagate fast in pure strains of induced mutations. Moreover, the central practical characters of mutant strains were studied: ①The first one was 7-I , which were wide blades (2.99±0.61)cm, grew faster (the length mean daily increase was 6.51 cm and it was 55.3% faster than control group when the length of the blades beyond 30 cm), was a high temperature resistant strain (it was grew normally at 29 ℃ and the resistant temperature was 2℃ more than control group ) and total phycobiliprotein content was high (104.858 mg·g-1 of dry sample, improved by 39.0%). ② The second one was 7-Ⅱ, which were narrow blades, had quick growth (the length mean daily increase was 4.26 cm and it was 58.7% faster than control group when the length of the blades beyond 30 cm), was a thin-blade strain (it was 22.5-27.5 μm in the middle part of the blade and was 29.6% thinner than control group) and total phycobiliprotein content was high (104.239 mg·g-1 of dry sample, improved by 39.4%) ③The third one was pigementation mutant, 3-I, which possessed of purple and thin blade(25.0-32.5 μm).④The yield of 7-I and 7-Ⅱ was 291.5-309.6 kg·666.6 m-2,which was 29%-37% higher than control group. The results laid a solid foundation for germplasm improvement and exploitation of fine variety of Porphyra haitanensis.

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陈昌生,徐燕,谢潮添.坛紫菜诱变育种的初步研究[J].水产学报,2008,32(3):327~334

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  • 收稿日期:2008-05-09
  • 最后修改日期:2008-05-09
  • 录用日期:2008-05-09
  • 在线发布日期: 2008-05-09
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