文章摘要
陈燕园,刘圣,何京,姚韩韩,林志华,董迎辉.浙江缢蛏群体糖原含量变化与gys基因多态性的关联分析[J].水产学报,2021,45(3):415~423
浙江缢蛏群体糖原含量变化与gys基因多态性的关联分析
Glycogen content fluctuation and association analysis with polymorphism of gys gene of Sinonovacula constricta in the Zhejiang population
投稿时间:2020-02-15  修订日期:2020-04-07
DOI:10.11964/jfc.20200212159
中文关键词: 缢蛏  糖原  糖原合酶  单核苷酸多态性位点 (SNP)
英文关键词: Sinonovacula constricta  glycogen  glycogen synthase  SNP
基金项目:现代农业产业技术体系专项(CARS-49);国家海洋水产种质资源库;浙江省农业新品种选育重大科技专项(2016C02055-9);宁波市“科技创新2025”重大专项(2019B10005)
作者单位E-mail
陈燕园 上海海洋大学水产科学国家级实验教学示范中心上海 201306
浙江万里学院浙江省水产种质资源高效利用技术研究重点实验室浙江 宁波 315100 
 
刘圣 浙江万里学院宁海海洋生物种业研究院浙江 宁海 315604  
何京 浙江万里学院浙江省水产种质资源高效利用技术研究重点实验室浙江 宁波 315100  
姚韩韩 浙江万里学院浙江省水产种质资源高效利用技术研究重点实验室浙江 宁波 315100  
林志华 浙江万里学院浙江省水产种质资源高效利用技术研究重点实验室浙江 宁波 315100
浙江万里学院宁海海洋生物种业研究院浙江 宁海 315604 
zhihua9988@126.com 
董迎辉 浙江万里学院浙江省水产种质资源高效利用技术研究重点实验室浙江 宁波 315100 dongyinghui118@126.com 
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中文摘要:
      为了探讨缢蛏糖原含量与糖原合酶基因(sc-gys)的特性及其用于分子标记辅助育种的可行性,实验检测了浙江缢蛏群体糖原含量的周年变化,克隆获得糖原合酶基因的cDNA全长序列,分析其在不同组织、不同月份的表达差异,并进一步筛选出与糖原含量相关的单核苷酸多态性 (single nucleotide polymorphism, SNP) 位点。结果显示,养殖缢蛏在2—6月糖原含量较高,4月达到最大值。sc-gys基因的cDNA全长为2 402 bp,开放阅读框为2 136 bp,编码711个氨基酸。荧光定量PCR结果显示,sc-gys基因主要在外套膜和足中表达,其不同月份的表达水平与糖原含量周年变化规律一致,4月表达量显著高于其他月份,表明该基因与糖原合成关系密切。在“甬乐1号”品种和台州野生群体sc-gys基因的外显子区共筛选到2个与糖原含量相关的SNP位点,且两位点之间没有连锁不平衡,优势基因型组合个体的糖原含量比群体均值提高11.8%,这些位点和优势基因型组合可为缢蛏糖原含量的遗传改良提供候选标记。
英文摘要:
      In order to explore the characteristics of glycogen content in Sinonovacula constricta, the glycogen synthase (sc-gys) gene and its feasibility in molecular marker-assisted breeding, the annual changes of glycogen content were detected in this study. The glycogen content in S. constricta was high between February to June and reached the maximum level in April during the annual cycle. The sc-gys gene has a full-length cDNA of 2 402 bp and an ORF of 2 136 bp, encoding 711 amino acids, with high similarity to orthologs of other animals showing conserved domain of Glycosyltransferase. The results of real-time quantitative PCR showed that sc-gys was mainly expressed in mantle and foot tissues. The expression level of sc-gys in April was significantly higher than that of other months, indicating its close relation to glycogen synthesis. Two SNP loci was tightly associated with glycogen content in the sc-gys exon region from "Yongle NO.1" new strain and wild population of Taizhou, and there was no linkage disequilibrium between the two SNP loci. Glycogen content of individuals with these two genotypes combinations was 11.8% higher than that of the population average. These two SNP loci and proper combination would provide candidate genetic markers for glycogen content genetic improvement of S. constricta.
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