文章摘要
何震晗,肖珊,王韶韶,陈慧芳,苏绍萍,赵永贞,杨春玲,曾地刚,朱威霖,陈秀荔,马华威,蒋伟明,刘青云,李强勇,彭敏.黄鳍棘鲷线粒体D-loop序列的遗传结构[J].水产学报,2021,45(3):345~356
黄鳍棘鲷线粒体D-loop序列的遗传结构
Genetic structure of D-loop sequence in Acanthopagrus latus
投稿时间:2019-12-25  修订日期:2020-05-29
DOI:10.11964/jfc.20191212113
中文关键词: 黄鳍棘鲷  D-loop序列  遗传多样性  遗传分化
英文关键词: Acanthopagrus latus  D-loop sequence  genetic diversity  genetic differentiation
基金项目:广西创新驱动发展专项(桂科AA17204088)
作者单位E-mail
何震晗 广西大学动物科学技术学院广西 南宁 530005
广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021 
 
肖珊 广西壮族自治区水产引育种中心广西 南宁 530031  
王韶韶 广西大学动物科学技术学院广西 南宁 530005  
陈慧芳 广西大学动物科学技术学院广西 南宁 530005
广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021 
 
苏绍萍 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021  
赵永贞 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021  
杨春玲 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021  
曾地刚 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021  
朱威霖 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021  
陈秀荔 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021  
马华威 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021  
蒋伟明 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021  
刘青云 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021  
李强勇 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021  
彭敏 广西壮族自治区水产科学研究院广西遗传育种与健康养殖重点实验室广西 南宁 530021 837969487@qq.com 
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中文摘要:
      为探明分布于我国华南沿海的黄鳍棘鲷群体的遗传多样性与遗传分化状况,实验采用线粒体控制区(D-loop)基因序列分析华南沿海的厦门、汕尾、阳江、海口、三亚、北海、钦州和防城港8个地理位置的黄鳍棘鲷群体的遗传多样性及群体遗传结构。结果显示,黄鳍棘鲷8个群体320条D-loop序列全长为947~958 bp。共检测到29个插入或缺失位点和210个变异位点,其中简约信息位点142个,单一变异位点68个;总体的变异位点、单倍型数、单倍型多样性(Hd)、平均核苷酸差异和核苷酸多样性(π)分别为210、268、0.998 43、14.790 65和0.015 70。聚类分析结果显示,8个群体被聚类为以琼州海峡分隔的东和西两个组群;8个群体间的遗传分化系数(FST)为−0.012 68~0.466 74,基因流(Nm)为0.571 26~∞。方差分析显示组群间、组群内群体间和群体内个体间的核苷酸遗传变异分别为33.42%、0.32%和66.26%。中性检验显示Tajima’s D为−1.694 77,Fu’s Fs为−23.683 39,表明华南沿海黄鳍棘鲷经历了种群扩张事件。研究表明,中国华南沿海黄鳍棘鲷群体遗传多样性比较丰富,根据研究结果可以以琼州海峡为分界分为东组群和西组群2个管理单位进行种质保护。
英文摘要:
      Acanthopagrus latus is a valuable marine fish and an important economic fish for marine aquaculture along the South China coast. In order to ascertain the genetic diversity and genetic differentiation status of A. latus populations distributed along the South China coast, in this study, the mitochondrial control region (D-loop) gene sequences were used to analyze the genetic diversity and population genetic structure of eight A. latus populations from the South China coast, including Xiamen, Shanwei, Yangjiang, Haikou, Sanya, and Beihai, Qinzhou, Qinzhou and Fangcheng. The results showed that 320 D-loop sequences from the eight A. latus populations were 947-958 bp in length. Totally, 29 insertion or deletion sites and 210 mutation sites (including 142 parsimony information sites and 68 single mutation sites) were detected in the D-loop sequences; overall mutation sites, haplotype number, and haplotype diversity (Hd), average nucleotide difference and nucleotide diversity (π) were 210, 268, 0.998 43, 14.790 65 and 0.01570, respectively. The results of cluster analysis showed that all populations were clustered into two groups, east and west separated by Qiongzhou Strait. The genetic differentiation coefficient (FST) between the eight populations was –0.01268-0.466 74, and gene flow (Nm) was 0.571 26~∞. The variance analysis showed that the genetic variation between groups, between populations within groups, and between individuals within populations were 33.42%, 0.32%, and 66.26%, respectively. Neutral tests showed that Tajima's D was –1.694 77 (P=0.010 00) and Fu 's Fs was –23.683 39 (P=0.00600), indicating that A. latus along the South China coast experienced a population expansion event. This study indicated that the genetic diversity of A. latus populations along the South China coast was relatively rich. This study suggested that A. latus populations can be divided into two management units by the Qiongzhou Strait, the east group and the west group for germplasm protection. Based on such monitoring results, some countermeasures and suggestions for the future restoration strategy were proposed so as to provide a theoretical basis for restoring and protecting the A. latus populations, and meanwhile it can also provide some scientific guidance for artificial breeding and value-added release in natural sea area.
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