文章摘要
姜涛,王成友,杜浩,张书环,刘洪波,危起伟,杨健.中华鲟硬组织微结构及微化学的特征探索[J].水产学报,2021,45(3):424~432
中华鲟硬组织微结构及微化学的特征探索
Investigation into the microstructure and microchemical characteristics of the hard tissues of Acipenser sinensis
投稿时间:2019-10-29  修订日期:2020-06-15
DOI:10.11964/jfc.20191012033
中文关键词: 中华鲟  耳石  背骨板  胸鳍条  微结构  微化学
英文关键词: Acipenser sinensis  otolith  dorsal scute  pectoral fin ray  microstructure  microchemistry
基金项目:中国长江三峡集团公司科研资助项目 (201603073)
作者单位E-mail
姜涛 中国水产科学研究院淡水渔业研究中心江苏 无锡 214081  
王成友 中国水产科学研究院长江水产研究所湖北 武汉 430223  
杜浩 中国水产科学研究院长江水产研究所湖北 武汉 430223  
张书环 中国水产科学研究院长江水产研究所湖北 武汉 430223  
刘洪波 中国水产科学研究院淡水渔业研究中心江苏 无锡 214081  
危起伟 中国水产科学研究院长江水产研究所湖北 武汉 430223 weiqw@yfi.ac.cn 
杨健 中国水产科学研究院淡水渔业研究中心江苏 无锡 214081 jiany@ffrc.cn 
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中文摘要:
      为了在中华鲟洄游生态学研究上有新突破,实验首次比较研究了中华鲟3种硬组织(耳石、背骨板和胸鳍条)的微结构和微化学特征。结果发现,耳石结构松散,存在多个耳砂(微晶球霰石球晶)颗粒,且每个颗粒均具有独立的核心;背骨板存在分层现象;胸鳍条结构较为致密,且均一。胸鳍条、耳石中生境元素Co/Ca、Sr/Ca和Ba/Ca比特征变化较为一致,而背板由于分层的原因与前二者有所差异。综合考虑非致死性采样、前处理难度、微结构组成以及生境元素生物积累等优势,笔者建议首选胸鳍条作为基于微化学分析来反演中华鲟洄游及生活史履历研究的最佳硬组织材料。
英文摘要:
      Chinese sturgeon (Acipenser sinensis) is an endemic, endangered and protected anadromous fish in China. This fish has been listed as a Category I nationally protected species in China and a critically endangered (CR) species of the International Union for Conservation of Nature (IUCN). Due to the restrictions by several special reasons of this species, e.g., long lifespan, complex migratory process, and precarious resource situation, it is quite difficult to objectively reveal the features of life history, and characteristics of distribution, and dynamics of habitat use (e.g., spawning/nursery site) by some traditional methods. Consequently, introducing more new innovative approaches is needed to achieve new breakthrough on research of migratory ecology and conservation for this species. In the present study, microchemical characteristics of three kinds of hard tissues of A. sinensis (i.e., otolith, dorsal scute, and pectoral fin ray) were studied comparatively by electron probe microanalyses (EPMA) and laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) with their microstructure. The results show that otoliths of A. sinensis are loose in structure and contain small sand-like microcrystalline vaterite spherulites with independent core areas. In constrast, the dorsal scute is a stratified tissue while the pectoral fin ray is a relatively compact and uniform tissue. Viewed from the microchemical results of the three hard tissues, the analytical accuracy across all samples was high for Mg, Ti, V, Mn, Cu, Zn, Co, Sr, Ba and Ca with RSD<10%. The z-Scores of habitat element ratios for Co/Ca, Sr/Ca and Ba/Ca in both pectoral fin ray (-0.97-2.16, -0.78-2.56, -0.96-2.41) and otolith (-1.7-0.74, -1.24-0.98, -1.09-1.09) are consistent, while those z-Scores in the dorsal scute (-1.95-2.32, -1.68-2.03, -1.62-2.68) are fluctuative due to its stratification. At the same time, the microchemical maps of Sr, Ca and Ba in these three hard tissues revealed that pectoral fin ray and dorsal scute were uniform color/concentration while different parts of otolith showed different colors/concentrations. Based on the aforementioned findings and we consider practical needs from no lethality, difficulty of sampling and sample preparation process, tissular microstructure composition, and environmental element bioaccumulation. In the present study, the microstructure and microchemical characteristics of three kinds of hard tissues of A. sinensis, i.e., otolith, dorsal scute, and pectoral fin ray, were studied comparatively. The present study suggested that the pectoral fin ray is the best hard tissue material for microchemical study for reconstructing the history of the migration and life cycle of A. sinensis.
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